Ultrasonic distance sensor improvement using a two-level neural network

This paper discusses the performance improvement that a neural network can provide to a contactless distance sensor based on the measurement of the time of flight (TOF) of an ultrasonic (US) pulse. The sensor, which embeds a correction system for the temperature effect, achieves a distance uncertainty (rms) of less than 0.5 mm over 0.5 m by using a two-level neural network to process the US echo and determine the TOF in the presence of environmental acoustic noise. The network embeds a "guard" neuron that guards against gross measurement errors, which would be possible in the presence of high environmental noise

Ultrasonic distance sensor improvement using a two-level neural network

This paper discusses the performance improvement that a neural network can provide to a contactless distance sensor based on the measurement of the time of flight (TOF) of an ultrasonic (US) pulse. The sensor, which embeds a correction system for the temperature effect, achieves a distance uncertainty (rms) of less than 0.5 mm over 0.5 m by using a two-level neural network to process the US echo and determine the TOF in the presence of environmental acoustic noise. The network embeds a "guard" neuron that guards against gross measurement errors, which would be possible in the presence of high environmental noise

Iron overload down-regulates the expression of the HIV-1 Rev cofactor eIF5A in infected T lymphocytes

Background Changes in iron metabolism frequently accompany HIV-1 infection. However, while many clinical and in vitro studies report iron overload exacerbates the development of infection, many others have found no correlation. Therefore, the multi-faceted role of iron in HIV-1 infection remains enigmatic. Methods RT-qPCR targeting the LTR region, gag, Tat and Rev were performed to measure the levels of viral RNAs in response to iron overload. Spike-in SILAC proteomics comparing i) iron-treated, ii) HIV-1-infected and iii) HIV-1-infected/iron treated T lymphocytes was performed to define modifications in the host cell proteome. Data from quantitative proteomics were integrated with the HIV-1 Human Interaction Database for assessing any viral cofactors modulated by iron overload in infected T lymphocytes. Results Here, we demonstrate that the iron overload down-regulates HIV-1 gene expression by decreasing the levels of viral RNAs. In addition, we found that iron overload modulates the expression of many viral cofactors. Among them, the downregulation of the REV cofactor eIF5A may correlate with the iron-induced inhibition of HIV-1 gene expression. Therefore, we demonstrated that eiF5A downregulation by shRNA resulted in a significant decrease of Nef levels, thus hampering HIV-1 replication. Conclusions Our study indicates that HIV-1 cofactors influenced by iron metabolism represent potential targets for antiretroviral therapy and suggests eIF5A as a selective target for drug development

Human-flood interactions in Rome over the past 150 years

Abstract. Throughout history, the socio-economic development of the city of Rome has been intertwined with the magnitude and frequency of flooding events from the Tiber, one of Italy's largest rivers. Ancient Rome mostly developed on the hills, while the Tiber's floodplain was mainly exploited for agricultural purposes. A few small communities did settle in the riparian areas of the Tiber, but they had a relatively peaceful relationship with the frequent occurrence of flooding events. Instead, numerous people live nowadays in modern districts in the Tiber's floodplain, unaware of their exposure to potentially catastrophic flooding. This research work aims to explore the dynamics of changing flood risk between these two opposite pictures of ancient and contemporary Rome. To this end, we carried out a socio-hydrological study by using long time series of hydrological (extreme flood events) and social (human population dynamics) processes, along with information about human interactions with the environment (flood defence structures). The historical analysis showed how human and water systems have been co-evolving over time, while being abruptly altered by the occurrence of an extreme flood event in 1870, just before Rome became the capital of a recently unified Italy. The outcomes of this study were then compared to the results of a socio-hydrological model simulating the dynamics emerging from the mutual shaping of floods and societies

Flow Duration Curve from Satellite:Potential of a Lifetime SWOT Mission

A flow duration curve (FDC) provides a comprehensive description of the hydrological regime of a catchment and its knowledge is fundamental for many water-related applications (e.g., water management and supply, human and irrigation purposes, etc.). However, relying on historical streamflow records, FDCs are constrained to gauged stations and, thus, typically available for a small portion of the world’s rivers. The upcoming Surface Water and Ocean Topography satellite (SWOT; in orbit from 2021) will monitor, worldwide, all rivers larger than 100 m in width (with a goal to observe rivers as small as 50 m) for a period of at least three years, representing a potential groundbreaking source of hydrological data, especially in remote areas. This study refers to the 130 km stretch of the Po River (Northern Italy) to investigate SWOT potential in providing discharge estimation for the construction of FDCs. In particular, this work considers the mission lifetime (three years) and the three satellite orbits (i.e., 211, 489, 560) that will monitor the Po River. The aim is to test the ability to observe the river hydrological regime, which is, for this test case, synthetically reproduced by means of a quasi-2D hydraulic model. We consider different river segmentation lengths for discharge estimation and we build the FDCs at four gauging stations placed along the study area referring to available satellite overpasses (nearly 52 revisits within the mission lifetime). Discharge assessment is performed using the Manning equation, under the assumption of a trapezoidal section, known bathymetry, and roughness coefficient. SWOT observables (i.e., water level, water extent, etc.) are estimated by corrupting the values simulated with the quasi-2D model according to the mission requirements. Remotely-sensed FDCs are compared with those obtained with extended (e.g., 20–70 years) gauge datasets. Results highlight the potential of the mission to provide a realistic reconstruction of the flow regimes at different locations. Higher errors are obtained at the FDC tails, where very low or high flows have lower likelihood of being observed, or might not occur during the mission lifetime period. Among the tested discretizations, 20 km stretches provided the best performances, with root mean absolute errors, on average, lower than 13.3%

Wetland selection, niche breadth and overlap in 10 duck species wintering in northern Campania (Italy)

Analysis of the selection index, the niche breadth and the niche overlap of 10 species of wintering ducks in 11 wetland areas of the Campania region between January 2003 and 2015 revealed that the distribution of the species in the examined sites was not casual. In fact, each species actively chose their wintering area. Using selection index, we were able to measure the species’ preferences and non-preferences for the 11 examined sites. By calculating Shannon-Wiener entropy and its normalized value (Evenness), we determined niche breadth; the Morisita index allowed us to check niche overlap

Citizens AND HYdrology (CANDHY): conceptualizing a transdisciplinary framework for citizen science addressing hydrological challenges

Widely available digital technologies are empowering citizens who are increasingly well informed and involved in numerous water, climate, and environmental challenges. Citizen science can serve many different purposes, from the "pleasure of doing science" to complementing observations, increasing scientific literacy, and supporting collaborative behaviour to solve specific water management problems. Still, procedures on how to incorporate citizens' knowledge effectively to inform policy and decision-making are lagging behind. Moreover, general conceptual frameworks are unavailable, preventing the widespread uptake of citizen science approaches for more participatory cross-sectorial water governance. In this work, we identify the shared constituents, interfaces, and interlinkages between hydrological sciences and other academic and non-academic disciplines in addressing water issues. Our goal is to conceptualize a transdisciplinary framework for valuing citizen science and advancing the hydrological sciences. Joint efforts between hydrological, computer, and social sciences are envisaged for integrating human sensing and behavioural mechanisms into the framework. Expanding opportunities of online communities complement the fundamental value of on-site surveying and indigenous knowledge. This work is promoted by the Citizens AND HYdrology (CANDHY) Working Group established by the International Association of Hydrological Sciences (IAHS)

Combination of chemotherapy and PD-1 blockade induces T cell responses to tumor non-mutated neoantigens

Here, we developed an unbiased, functional target-discovery platform to identify immunogenic proteins from primary non-small cell lung cancer (NSCLC) cells that had been induced to apoptosis by cisplatin (CDDP) treatment in vitro, as compared with their live counterparts. Among the multitude of proteins identified, some of them were represented as fragmented proteins in apoptotic tumor cells, and acted as non-mutated neoantigens (NM-neoAgs). Indeed, only the fragmented proteins elicited effective multi-specific CD4+ and CD8+ T cell responses, upon a chemotherapy protocol including CDDP. Importantly, these responses further increased upon anti-PD-1 therapy, and correlated with patients’ survival and decreased PD-1 expression. Cross-presentation assays showed that NM-neoAgs were unveiled in apoptotic tumor cells as the result of caspase-dependent proteolytic activity of cellular proteins. Our study demonstrates that apoptotic tumor cells generate a repertoire of immunogenic NM-neoAgs that could be potentially used for developing effective T cell-based immunotherapy across multiple cancer patients

Disease-Modifying Therapies and Coronavirus Disease 2019 Severity in Multiple Sclerosis

Objective: This study was undertaken to assess the impact of immunosuppressive and immunomodulatory therapies on the severity of coronavirus disease 2019 (COVID-19) in people with multiple sclerosis (PwMS). Methods: We retrospectively collected data of PwMS with suspected or confirmed COVID-19. All the patients had complete follow-up to death or recovery. Severe COVID-19 was defined by a 3-level variable: mild disease not requiring hospitalization versus pneumonia or hospitalization versus intensive care unit (ICU) admission or death. We evaluated baseline characteristics and MS therapies associated with severe COVID-19 by multivariate and propensity score (PS)-weighted ordinal logistic models. Sensitivity analyses were run to confirm the results. Results: Of 844 PwMS with suspected (n = 565) or confirmed (n = 279) COVID-19, 13 (1.54%) died; 11 of them were in a progressive MS phase, and 8 were without any therapy. Thirty-eight (4.5%) were admitted to an ICU; 99 (11.7%) had radiologically documented pneumonia; 96 (11.4%) were hospitalized. After adjusting for region, age, sex, progressive MS course, Expanded Disability Status Scale, disease duration, body mass index, comorbidities, and recent methylprednisolone use, therapy with an anti-CD20 agent (ocrelizumab or rituximab) was significantly associated (odds ratio [OR] = 2.37, 95% confidence interval [CI] = 1.18-4.74, p = 0.015) with increased risk of severe COVID-19. Recent use (<1 month) of methylprednisolone was also associated with a worse outcome (OR = 5.24, 95% CI = 2.20-12.53, p = 0.001). Results were confirmed by the PS-weighted analysis and by all the sensitivity analyses. Interpretation: This study showed an acceptable level of safety of therapies with a broad array of mechanisms of action. However, some specific elements of risk emerged. These will need to be considered while the COVID-19 pandemic persists